Disaccahrides-Based Cryo-Formulant Effect on Modulating Phospho/Mitochondrial Lipids and Biological Profiles of Human Leukaemia Cells

Background/Aims: The use of novel cryo-additive agents to increase cell viability post-cryopreservation is paramount improve future based-therapy treatments. We aimed establish the Human Leukemia (HL-60) cells lipidomic and biological patterns when cryo-preserved in DMSO alone with 300 µM Nigerose (Nig), 200 Salidroside (Sal) or a combination Nig (150 µM) Sal (100 µM). Methods: HL-60 were pre-incubated Nig/Sal prior, during post cryopreservation, subjected global analysis. Malondialdeyhde (MDA), released lactate dehydrogenase (LDH) reactive oxygen scavenger (ROS) measurements also carried out evaluate levels lipid peroxidation cytotoxicity. Results: Cryopreserving provided optimal protection against unsaturated fatty acid oxidation. Post-thaw, cellular phospholipids mitochondrial cardiolipins increased by as ratio saturated acids 2.08 +/- 0.03 0.95 0.09 folds respectively comparison cryopreserved (0.49 0.05 0.86 0.10 folds). presence + combined significantly reduced relative pre-cryopreservation (10.91 2.13 nmole) compared (17.1 3.96 nmole). cytotoxicity post-thaw (0.0128 0.00182 mU/mL) (0.0164 0.00126 mU/mL). ROS prior cryopreservation HL-60. Conclusion: Overall, establishment corresponding profiles showed an improved cryo-formulation protecting the, inner membrane, components (i. e. Cardiolipins) total phospholipids.